A single-tube, 20-color panel effectively detects aberrant cells in acute myeloid leukemia.

Abstract Objective: Multiparameter flow cytometry is widely and routinely used in acute myeloid leukemia (AML) diagnosis the residual disease detection monitoring. We have developed a single-tube, 20-color panel for AML analysis that uses Cytek® spectral cytometer with increased reagent sample efficiency. Methods: Blood bone marrow samples were stained panel, acquired, analyzed using SpectroFlo® software. The resolution of each marker was compared between single stain versus full on same samples. analytical precision cell populations interest antibody cocktail stability assessed. limit (LOD) lower quantification (LLOQ) assay determined limiting dilution method. Results: fully comparable to single-color Normal aberrant clearly identified coefficient variation (CV) percentage defined replicate runs all less than 25%. stable fridge at least 14 days. Our data showed easily achieved 0.01% cells AML, higher minimum required sensitivity 0.1%. Conclusions: Cytek’s demonstrated an effective, high approach can be testing, including identifying characterizing normal cells, immunophenotypic classification minimal evaluation translation research.